Frequently Asked Questions

How do I submit samples for Microbial Identification?

    1. Where possible, submit pure cultures of the organism that is to be identified.
      1. Perform all subsequent procedures in a sterile environment (i.e. Biosafety Cabinet) and make use of aseptic techniques at all times.
      2. Streak out an individual colony of the organism of interest onto an enrichment agar and incubate for 18-24 hours.
      3. If several colony morphologies are observed after incubation, streak out a colony of the organism of interest again. Repeat this step until a pure culture is obtained.
      4. If the organism is in liquid media, use spread-plate method to isolate the organisms. Should there be more than one colony morphology observed, proceed as per step b and c until pure cultures are obtained.
      5. Parafilm the pure culture plates to prevent cross contamination from other samples or from the environment.
    2. If pure cultures are not possible, submit the actual plate, broth, or solution. Seal the plates, tubes or bottles with Parafilm.

    3. Label all samples with their respective sample ID's using stickers or with permanent marker (cover the label with clear tape to ensure that the label is not erased should it come into contact with solvents).

    4. When submitting liquid samples, place each sample in an individual bag and seal to preserve the integrity of each sample should the other samples leak.

    5. Fill out the Test Sample Submission Form (TSSF). Indicate the individual Sample ID’s under Test Sample Name. The Sample ID’s indicated on the TSSF will be reflected in the Test Report.

    6. Send the samples in ice boxes with ice packs to ensure that the samples are kept cold. Submit the TSSF along with the samples. Samples must arrive at Charles River Singapore by Monday afternoon for the samples to be included in the scheduled weekly testing.

How do I submit samples for Mycoplasma Detection?

    1. Aseptically transfer 5-10ml of cell culture supernatant into a sterile centrifuge tube.

    2. Seal the tube with Parafilm and label the tube with the sample ID using stickers or with permanent marker (cover the label with clear tape to ensure that the label is not erased should it come into contact with solvents).

    3. If more than one sample is to be submitted, place each sample in separate bags to preserve the integrity of each sample should the other samples leak.

    4. Fill out the Test Sample Submission Form (TSSF). Indicate the individual Sample ID’s under Test Sample Name. The Sample ID’s indicated on the TSSF will be reflected in the Test Report.

    5. Send the samples in ice boxes with ice packs to ensure that the samples are kept cold. Submit the TSSF along with the samples. Samples must arrive at Charles River Singapore by Thursday morning for the samples to be included in the scheduled weekly testing.

How do I submit water samples for testing?

  1. Examine the sample bottle for cracks, a missing seal, or other signs that its sterility may be compromised. If any of these indications are found, discard the container and use a suitable one.

  2. Collection of water for endotoxin and microbiological tetsing should be performed in an aseptic manner:
    1. Inspect the outside of the faucet. Remove debris and sterilize the faucet outlet by swabbing with a disinfecting wipe.

    2. Allow the water to run for 2 minutes before collection, this will help to remove stagnant water from the system. Before taking the sample, reduce the tap flow rate to ensure that no splashing occurs as the container is filled. Do not adjust the flow rate while taking the sample.

    3. While holding the sample container at the base, remove the seal around the cap before attempting to open the bottle.

    4. Remove the cap with the free hand. Be careful NOT TO TOUCH the inside of the bottle cap or bottle lip. Continue to hold the cap in one hand with the inside facing down while the bottle is being filled.

      Note:

      • Do not touch the interior of the cap or lay it down.
      • Do not breathe on the bottle or cap.
      • Do not rinse the bottle.
      • Do not allow the bottle to overflow.
    5. Carefully replace the cap.

  3. Samples shall be kept in a refrigerator or cooler with ice packs to maintain a temperature of 4°C until delivered to the lab. Samples should not be frozen.

  4. When submitting liquid samples, place each sample in an individual bag and seal to preserve the integrity of each sample should the other samples leak.

  5. Fill out the Test Sample Submission Form (TSSF). Indicate the individual Sample ID’s under Test Sample Name. The Sample ID’s indicated on the TSSF will be reflected in the Test Report.

  6. Transport the sample to the laboratory as soon as possible. Check ahead with Charles River Singapore in advance when your samples are to be delivered to ensure meeting the recommended window period for water testing.

How do I submit samples for EO and ECH residue analysis?

  1. For samples that are submitted to Charles River Singapore for the first time, at least three trial samples of each product should be submitted for protocol development and screening for interfering compounds. The trial samples should not be sterilized and do not require dry ice shipping. 

  2. After being removed from the aeration area, the test samples should be shipped to Charles River Singapore on dry ice as soon as possible. If the samples cannot be shipped immediately, store the samples in a freezer (<-20°C).

  3. In addition to the primary packaging, samples should be individually sealed with a non-breathable material, for example, Aluminium foil pouch (preferred) or PVC plastic bag. This is to minimize additional aeration during shipment, as well as to avoid cross-sample interferences especially when samples from different aeration days are shipped together. 

  4. The dry ice in the shipment should be sufficient so that dry ice is still present by the time the container is opened in Charles River Singapore laboratory. For international shipping, please cater for an additional 1-2 shipping days as in some rare cases shipments containing dry ice may require additional processing time at the Customs.

 


Limulus Amebocyte Lysate (LAL)
Reagents and Accessory Products

and Authorised Agent for
Charles River Biopharmaceutical Services

Contact Us

Charles River Singapore
33 Ubi Avenue 3, #06-14,
Vertex (Tower B),
Singapore 408868

Tel: +65 6742 6007 
Fax: +65 6742 5669


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